Sequence exploration reveals information bias among molecular markers used in phylogenetic reconstruction for Colletotrichum species

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dc.contributor.authorRampersad, Sephra N
dc.contributor.authorHosein, Fazeeda N
dc.contributor.authorCarrington, Christine VF
dc.date.accessioned2014-10-21T14:59:32Z
dc.date.available2014-10-21T14:59:32Z
dc.date.issued2014-10-17
dc.date.updated2014-10-21T14:59:33Z
dc.description.abstractAbstract The Colletotrichum gloeosporioides species complex is among the most destructive fungal plant pathogens in the world, however, identification of isolates of quarantine importance to the intra-specific level is confounded by a number of factors that affect phylogenetic reconstruction. Information bias and quality parameters were investigated to determine whether nucleotide sequence alignments and phylogenetic trees accurately reflect the genetic diversity and phylogenetic relatedness of individuals. Sequence exploration of GAPDH, ACT, TUB2 and ITS markers indicated that the query sequences had different patterns of nucleotide substitution but were without evidence of base substitution saturation. Regions of high entropy were much more dispersed in the ACT and GAPDH marker alignments than for the ITS and TUB2 markers. A discernible bimodal gap in the genetic distance frequency histograms was produced for the ACT and GAPDH markers which indicated successful separation of intra- and inter-specific sequences in the data set. Overall, analyses indicated clear differences in the ability of these markers to phylogenetically separate individuals to the intra-specific level which coincided with information bias.
dc.description.versionPeer Reviewed
dc.identifier.citationSpringerPlus. 2014 Oct 17;3(1):614
dc.identifier.urihttp://dx.doi.org/10.1186/2193-1801-3-614
dc.identifier.urihttps://hdl.handle.net/2139/39145
dc.language.rfc3066en
dc.rights.holderSephra N Rampersad et al.; licensee BioMed Central Ltd.
dc.titleSequence exploration reveals information bias among molecular markers used in phylogenetic reconstruction for Colletotrichum species
dc.typeJournal Article

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