Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro

dc.contributor.authorCamargo Garbin, Livia
dc.contributor.authorMcIlwraith, C. W
dc.contributor.authorFrisbie, David D
dc.date.accessioned2019-11-03T04:21:12Z
dc.date.available2019-11-03T04:21:12Z
dc.date.issued2019-11-01
dc.date.updated2019-11-03T04:21:13Z
dc.description.abstractAbstract Background Platelet-rich plasma (PRP) as well as other platelet-derived products have been used as a potential disease-modifying treatment for musculoskeletal diseases, such as osteoarthritis (OA). The restorative properties of such products rely mainly on the high concentrations of growth factors, demonstrating encouraging results experimentally and clinically. Yet, the autologous blood-derived nature of the PRP product lead to limitations that precludes it’s widespread use. The main limitations for PRP use are; product variability, the need for minimum laboratory settings in most cases, and the need for storage at low temperatures to preserve its properties. Based on these limitations, the objective of this study was to investigate an allogeneic off-the-shelf platelet lysate (PL) in cartilage exposed to interleukin 1β (IL-1β). For this purpose, blood and cartilage were harvested from eight skeletally mature and healthy horses. Blood was processed into PL aliquots and divided into three groups (Frozen, Freeze-dried and Filtered freeze-dried), used in autologous and allogeneic conditions and in three different concentrations (1.5, 3 and 6-fold). Different PL preparations were then applied in cartilage culture with interleukin-1 beta and cultured for 10 days. Cartilage and media samples were collected and analyzed for total GAG and 35SO4-labeled GAG content. Results No significant differences between the controls and PL groups in cartilage and media were demonstrated. The effects of PL on cartilage matrix were concentration dependent and intermediate concentrations (3-fold) in PL showed increased 35SO4-labelled GAG in cartilage. Conclusion In conclusion, the allogeneic freeze-dried PL presented equivalent effects compared to frozen autologous PL. Intermediate platelet concentration on average demonstrated improved results, demonstrating less GAG loss compared to other concentrations.
dc.identifier.citationBMC Veterinary Research. 2019 Nov 01;15(1):386
dc.identifier.urihttps://doi.org/10.1186/s12917-019-2118-z
dc.identifier.urihttps://hdl.handle.net/2139/48234
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.titleEvaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro
dc.typeJournal Article

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