ELECTRON MICROSCOPY UNIT Klaus W. Wolf, PhD – Head of Unit WORK OF THE UNIT During the 2008/2009 academicyear, the Electron Microscopy (EM) Unit, Mona has continued conducting research, teaching and rendering assistance to Researchers, Postgraduate-, Undergraduate -, High School- students and other interest groups. These persons either got results from, or understanding of the uses and operations of any or all of the following: Transmission Electron Microscopy, Scanning Electron Microscopy, Optical Microscopy (OM), Macro-Photography (MP). Table 1 lists the main services provided during the said academic year. Table 1 Main services provided by the EM Unit during the 2008/2009 academic year. Specimen Technique Institution Acetate peels of rock surface OM Dept. of Geography & Geology Ackee root tips OM Biotechnology Centre Flower of Opuntia sp. MP Dept. of Life Sciences Fungi MP Dept. of Life Sciences Larvae of Citrus Butterfly MP Dept. of Life Sciences Mammalian Tissue OM Dept. of Basic Med. Sci. - Pharmacology Microscopic fossils OM Dept. of Geography & Geology Rat Liver OM Dept. of Basic Med. Sci. - Anatomy Rat liver and aorta OM Dept. of Basic Med. Sci. - Pharmacology Rat pancreas OM Dept of Basic Med. Sci. - Physiology Rock sections OM 13 different students - Dept. of Geography & Geology Soil particles & Leaves OM Dept. of Geography & Geology Spiders OM, MP Dept. of Geography & Geology Tissue culture in plants OM Biotechnology Centre 423 Lectures and laboratory sessions in Electron Microscopy were conducted in the Virology course “BL38A”, from the Department of Life Sciences and the Medical Physics and Bioengineering course “P29A” from the department of Physics. Lectures were conducted in the graduate course Research Methods for Biologists “BL60E”, from the Department of Life Sciences and a laboratory session for the graduate course Research Methods from the Department of Chemistry. Posters, exhibits and demonstrations were contributed to Research Day activities on the Mona campus of the University and a poster and exhibits were contributed to the UWI Fair on the Western Campus in Montego Bay. Collaborations continue with Zoologists from the University of Salzburg (Austria) and Wittenberg College (USA) on Ciliates in tank bromeliads and Kinetics of spermatogenesis in lizards, respectively. Research in progress Gamete structure and development in insects, using various microscopic techniques. Effects of chemicals on red blood cells. Refereed Publications * Gribbins, K. M., Rheubert, J. L., Poldemann, E. H., Collier, M. H., Wilson, B., Wolf, K. W. Continuous spermatogenesis and the germ cell development strategy within the testis of the Jamaican Gray Anole, Anolis lineatopus. Theriogenology 72: 484-492, 2009 * Foissner, W. and Wolf, K.W. Morphology and ontogenesis of Platyophrya bromelicola nov. spec., a new macrostome-forming colpodid (Portists, Ciliophora) from tank bromeliads of Jamaica. Eur. J. Protistol. 45: 87-97, 2009. * Oertel, A., Wolf, K.W., Al-Rasheid, K. and Foissner, W. Revision of the genus Coriplites Foissner, 1988 (Ciliophora: Haptorida), with description of Apocoriplites nov. gen. and three new species. Acta Protozool., 47: 231-46, 2008 424 With a staff complement of two, the per capita publication = 1.5. Targets In the 2009/2010 academic year, the EM Unit aims to: Pursue joint venture funding that will assist in the acquisition of a new scanning electron microscope; Continue its involvement in teaching and research; Continue its core function of assistance to researchers and exposing interest groups to the benefits and techniques of electron microscopy. 425