Sequence exploration reveals information bias among molecular markers used in phylogenetic reconstruction for Colletotrichum species

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dc.contributor.author Rampersad, Sephra N
dc.contributor.author Hosein, Fazeeda N
dc.contributor.author Carrington, Christine VF
dc.date.accessioned 2014-10-21T14:59:32Z
dc.date.available 2014-10-21T14:59:32Z
dc.date.issued 2014-10-17
dc.identifier.citation SpringerPlus. 2014 Oct 17;3(1):614
dc.identifier.uri http://dx.doi.org/10.1186/2193-1801-3-614
dc.identifier.uri http://hdl.handle.net/2139/39145
dc.description.abstract Abstract The Colletotrichum gloeosporioides species complex is among the most destructive fungal plant pathogens in the world, however, identification of isolates of quarantine importance to the intra-specific level is confounded by a number of factors that affect phylogenetic reconstruction. Information bias and quality parameters were investigated to determine whether nucleotide sequence alignments and phylogenetic trees accurately reflect the genetic diversity and phylogenetic relatedness of individuals. Sequence exploration of GAPDH, ACT, TUB2 and ITS markers indicated that the query sequences had different patterns of nucleotide substitution but were without evidence of base substitution saturation. Regions of high entropy were much more dispersed in the ACT and GAPDH marker alignments than for the ITS and TUB2 markers. A discernible bimodal gap in the genetic distance frequency histograms was produced for the ACT and GAPDH markers which indicated successful separation of intra- and inter-specific sequences in the data set. Overall, analyses indicated clear differences in the ability of these markers to phylogenetically separate individuals to the intra-specific level which coincided with information bias.
dc.title Sequence exploration reveals information bias among molecular markers used in phylogenetic reconstruction for Colletotrichum species
dc.type Journal Article
dc.date.updated 2014-10-21T14:59:33Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder Sephra N Rampersad et al.; licensee BioMed Central Ltd.


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